Value 
400
µm^2/sec

Organism 
Lizard Anolis carolinensis 
Reference 
Land BR, Harris WV, Salpeter EE, Salpeter MM. Diffusion and binding constants for acetylcholine derived from the falling phase of miniature endplate currents. Proc Natl Acad Sci U S A. 1984 Mar81(5):15948. abstractPubMed ID6584895

Method 
"[Researchers] recorded MEPCs [miniature endplate currents] by voltage clamp from the intercostal
muscle of the lizard Anolis carolinensis, using experimental
techniques as in the first two papers of this series (1, 2) with
minor modifications." 
Comments 
"[Researchers] find diffusion to be 4×10^6cm^2/sec, slightly slower than free diffusion, forward binding to be 3.3×10^7M^1 s^1, and the distance from an average release site to the nearest exit from the cleft to be 1.6µm...The diffusion rate constant is somewhat smaller than that
for free diffusion [given as 610×10^6cm^2/Sec (refs 2830)]." 
Entered by 
Uri M 
ID 
111076 