Concentration of nucleic acids and proteins in strain k-12 grown on MGM and MGM+GB

Value 220 mg/ml
Organism Bacteria Escherichia coli
Reference Cayley S, Record MT Jr. Roles of cytoplasmic osmolytes, water, and crowding in the response of Escherichia coli to osmotic stress: biophysical basis of osmoprotection by glycine betaine. Biochemistry. 2003 Nov 4 42(43):12596-609. p.12604 right column bottom paragraph and p.12605 fig.7APubMed ID14580206
Method The amount of RNA in the supernatant was quantified by the orcinol assay (Herbert et al., 1971 Methods Microbiol. 513, 209-234.) using total yeast RNA as a standard. The total amount of protein in cells was determined by the method of Lowry et al. (1951, PMID 14907713) using BSA as a standard.
Comments At cytoplasmic water amount of 110 µmol/mg of DW [characteristic of cells grown in MBM (and MBM+GB) at the optimal growth osmolality of ~0.3 Osm]. MBM=MOPS-buffered minimal glucose medium. GB=glycine betaine
Entered by Uri M
ID 105904