Radial growth rate of colony

Value 0.013 µm/sec
Organism Bacteria Escherichia coli
Reference Hallatschek O, Hersen P, Ramanathan S, Nelson DR. Genetic drift at expanding frontiers promotes gene segregation. Proc Natl Acad Sci U S A. 2007 Dec 11 104(50):19926-30. p. 19927 right column, top paragraphPubMed ID18056799
Method The two (immotile) strains of E. coli were grown overnight in separate liquid cultures at 37°C in rich growth medium (LB) supplemented with Ampicillin. After tenfold dilution in fresh medium, we mixed them to obtain cultures with different proportions of the two kinds of cells. Droplets of different sizes were then pipetted on LB-agar plates (1.5% Agar) with Ampicillin, and grown at 37°C for up to 84 h. Population growth and mutant distribution were monitored at regular time intervals by means of a stereo microscope.
Comments Note-Value of 0.023µm/sec for radial colony growth rate of a gram positive bacterium is given by Nithyaja et al., Kinetics of Bacterial Colony Growth by Laser Induced Fluorescence, Laser Physics, 2009, Vol. 19, No. 3, pp. 468–472. p. 469 right column, bottom sentence, fig. 4
Entered by Uri M
ID 105542