DNA replication rate of Klenow fragment of DNA Polymerase I

Value 13.5 bp/sec
Organism Bacteria Escherichia coli
Reference Goel A, Ellenberger T, Frank-Kamenetskii MD, Herschbach D. Unifying themes in DNA replication: reconciling single molecule kinetic studies with structural data on DNA polymerases. J Biomol Struct Dyn. 2002 Feb19(4):571-84 p.576 caption of fig.7 and p.577 caption of fig.10PubMed ID11843619
Primary Source Maier B, Bensimon D, Croquette V. Replication by a single DNA polymerase of a stretched single-stranded DNA. Proc Natl Acad Sci U S A. 2000 Oct 24 97(22):12002-7PubMed ID11050232
Comments The Klenow fragment is a large protein fragment produced when DNA polymerase I from E. coli is enzymatically cleaved by the protease subtilisin.
Entered by Uri M
ID 103999