Association constant for codon recognition step of tRNA selection

Value 190 sec^-1
Organism Bacteria Escherichia coli
Reference Hani S. Zaher and Rachel Green, Fidelity at the Molecular Level: Lessons from Protein Synthesis. Cell Volume 136, Issue 4, 20 February 2009, Pages 746-762PubMed ID19239893
Primary Source T. Pape, W. Wintermeyer and M.V. Rodnina, Complete kinetic mechanism of elongation factor Tu-dependent binding of aminoacyl-tRNA to the A site of the E-coli ribosome, EMBO J. 17 (1998), pp. 7490–7497.PubMed ID9857203
Method pre-steady-state kinetic experiments. Rate constants monitored by fluorescence changes in labeled tRNAs
Comments The initial selection of tRNA is comprised of initial binding step, which is independent of the codon/anti codon interaction, Coding recognition step, in which codon/anticodon recognition occurs and a GTPase activation step. Constant is for coding recognition of (EF-Tu+ GTP+ aa-tRNA) ternary complex and A site of ribosome. This value is similar both for cognate (correct) codons and near cognate (that differ by one nucleotide) codons
Entered by Uri M
ID 103472