Rate in which ribosomal release factor mistakes a sense codon for a nonsense codon

Value 1E-05 misread sense codons/correctly read sense codons
Organism Bacteria Escherichia coli
Reference Hani S. Zaher and Rachel Green, Fidelity at the Molecular Level: Lessons from Protein Synthesis. Cell Volume 136, Issue 4, 20 February 2009, Pages 746-762PubMed ID19239893
Primary Source F. Jorgensen, F.M. Adamski, W.P. Tate and C.G. Kurland, Release factor-dependent false stops are infrequent in Escherichia coli, J. Mol. Biol. 230 (1993)PubMed ID8450549
Comments In contrast to tRNA selection that depends on the exact geometry of nucleotide base pairing to determine selectivity, release factors depend on RNA-protein interactions to discriminate between highly similar sense and nonsense codons. Interestingly, the accuracy of this reaction is more than one order of magnitude greater than that for tRNA selection. Note-Dong et al., 1995 PMID: 7752224 p.556 left column 1st paragraph give a processivity loss of 2×10^-4 per codon.
Entered by Uri M
ID 103468