Quantitative measurements of synaptic vesicle [SV] Proteins and Lipids

Range Table - link
Organism Rat Rattus norvegicus
Reference Takamori S et al., Molecular anatomy of a trafficking organelle. Cell. 2006 Nov 17 127(4):831-46. DOI: 10.1016/j.cell.2006.10.030 p.837 figure 1PubMed ID17110340
Primary Source See refs beneath table
Method P.836 right column bottom paragraph: "To construct a molecular model of a prototypic SV, [investigators] first calculated the average copy number per vesicle of the proteins quantified above (see Figure 1) based on the known molecular mass, Avogadro's number, and the average protein content per vesicle (Table 2, see also Table S2)."
Comments P.836 left column 2nd paragraph: "Next, [investigators] performed quantitative determinations of more than a dozen major SV proteins. Quantitation was performed by either western blotting or dot blotting, using purified proteins as standards. Figure 1A shows an example of such a determination, and Figure 1B summarizes the results for all analyzed proteins, including a few data from previous publications. Synaptophysin, synaptobrevin 2 (also referred to as VAMP2), synaptotagmin 1, and synapsin I are among the most abundant proteins, in very good agreement with earlier results (primary sources Goelz et al., 1981, Walch-Solimena et al., 1995)."
Entered by Uri M
ID 103353