Concentration of Raf in HeLa cells

Value 0.0057 µM Range: Table - link µM
Organism Mammalian tissue culture cell
Reference Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes. Fujioka A, Terai K, Itoh RE, Aoki K, Nakamura T, Kuroda S, Nishida E, Matsuda M. J Biol Chem. 2006 Mar 31 281(13):8917-26PubMed ID16418172
Primary Source Schoeberl B, Eichler-Jonsson C, Gilles ED, Müller G. Computational modeling of the dynamics of the MAP kinase cascade activated by surface and internalized EGF receptors. Nat Biotechnol. 2002 Apr20(4):370-5 Table - link PubMed ID11923843
Method (Primary source) A computational model that offers an integrated quantitative, dynamic, and topological representation of intracellular signal networks, based on known components of epidermal growth factor (EGF) receptor signal pathways.
Comments This value is from table link in reference. It seems to be taken from primary source table link value of 4×10^4 Raf/cell given in Primary source table link. However, if calculated according to cell volume of 1 pL (note under ref table link) one arrives at [(4×10^4<Raf/cell>/~6×10^23)<Raf/mole>]/10^12<liter/cell>=~0.067µM, an order of magnitude greater than above value. Above value is half of Raf conc. of 0.013µM given by ref (FRET method), BNID 100854. In silico (performed on computer) kinetic simulation models of the RAS/ERK MAPK pathway, while describing it very nicely, have produced astonishingly different parameters. The aim of study in ref was to collect and evaluate the parameters for the development of a kinetic simulation model for Ras/ERK MAPK cascades.
Entered by Cellina Cohen-Saidon
ID 100890