Summary of mutations detected in control and extreme cells

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Gilad Yaakov, David Lerner, Kajetan Bentele, Joseph Steinberger and Naama Barkai, Coupling phenotypic persistence to DNA damage increases genetic diversity in severe stress, Nature Ecology & Evolution 1, Article number: 0016 (2017) pdf link doi:10.1038/s41559-016-0016 p.5 table 1
Primary Source [25] Lynch, M. et al. A genome-wide view of the spectrum of spontaneous mutations in yeast. Proc. Natl Acad. Sci. USA 105, 9272–9277 (2008). doi: 10.1073/pnas.0803466105. [27] Serero, A., Jubin, C., Loeillet, S., Legoix-Ne, P. & Nicolas, A. G. Mutational landscape of yeast mutator strains. Proc. Natl Acad. Sci. USA 111, 1897–1902 (2014). DOI: 10.1073/pnas.1314423111PubMed ID18583475, 24449905
Method Abstract: "[Investigators] report that spontaneous DNA damage triggers persistence in Saccharomyces cerevisiae by activating the general stress response, providing protection against a range of harsh stress and drug environments."
Comments P.5 right column bottom paragraph: "Indels or SVs (structural variations) were found in 26 of the 412 extreme samples, compared with 6 out of the 315 control samples (Table 1)." P.6 left column 2nd paragraph: "The frequencies of mutations in [investigators’] control samples are consistent with literature values (Table 1 and Supplementary Information) (primary sources 25, 27, ref 26). Estimating the mutation frequency of extreme cells is less straightforward, since cells are extreme only transiently, and their division time, while being extreme, is greatly prolonged (Fig. 2e). Lower and upper bounds are obtained, however, by respectively assuming that extreme samples differ from the control cells in all 23–25 divisions, or that they differ in only the last extreme division (Table 1). The extreme dynamics [they] describe suggest that this mutation frequency is closer to the upper bound, namely that a division which triggers cells to become extreme has ~5% chance of generating an indel or an SV." See notes beneath table
Entered by Uri M
ID 113263