Biomass constituents of protein, carbohydrate, lipids, RNA & DNA

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Lange HC, Heijnen JJ. Statistical reconciliation of the elemental and molecular biomass composition of Saccharomyces cerevisiae. Biotechnol Bioeng. 2001 Nov 5 75(3):334-44. p.336 table IPubMed ID11590606
Primary Source Kockova´-Kratochvílova´ A. 1990. Yeast and yeast-like organisms. New York, VCH Publisher.
Method Abstract: "A systematic mathematical procedure capable of detecting the presence of a gross error in the measurements and of reconciling connected data sets by using the maximum likelihood principle is applied to the biomass composition data of yeast. The biomass composition of Saccharomyces cerevisiae grown in a chemostat under glucose limitation was analyzed for its elemental and for its molecular composition. Both descriptions initially resulted in conflicting results concerning the elemental composition, molecular weight, and degrees of reduction. The application of the statistical reconciliation method, based on elemental balances and equality relations, is used to obtain a consistent biomass composition."
Comments P.336 right column 2nd paragraph: "The following premises are made for the macromolecule composition of yeast: their composition is independent of the growth rate. The carbohydrates can be described as polyhexoses with infinite linear chains. For the nucleic acids, a guanine and cytidine content of each 20% is assumed. Lipids are taken as 60% fat and 40% phospholipids the acyl groups are made up of palmitoleic, oleic, palmitic, and stearic acids in a ratio of 44:17:14:10, as reported for S. cerevisiae grown on synthetic media by Kockova´ (Kockova´-Kratochvílova´, 1990). One acyl group is substituted in the phospholipids with an average of 23, 62, and 16% of ethanolamine, choline, and serine, respectively, bound through a phosphate group. The protein composition was derived from the experimental analysis of the amino acids. The resulting elemental composition of each macromolecule is given in Table I."
Entered by Uri M
ID 112799