G-actin concentration at the lamellipodium tip in 3T3 fibroblast

Range ~8 μM
Organism Mouse Mus musculus
Reference Koestler, S.A., K. Rottner, F. Lai, J. Block, M. Vinzenz, and J.V. Small. 2009. F- and G-actin concentrations in lamellipodia of moving cells. PLoS ONE. 4:e4810. doi:10.1371/journal.pone.0004810 p.1 right column top paragraphPubMed ID19277198
Primary Source [19] Abraham VC, Krishnamurthi V, Taylor DL, Lanni F (1999) The actin-based nanomachine at the leading edge of migrating cells. Biophys J 77: 1721–1732. DOI:10.1016/S0006-3495(99)77018-9PubMed ID10465781
Method Primary source abstract: "Two fundamental parameters of the highly dynamic, ultrathin lamellipodia of migrating fibroblasts have been determined-its thickness in living cells (176 +/- 14 nm), by standing-wave fluorescence microscopy, and its F-actin density (1580 +/- 613 microm of F-actin/microm(3)), via image-based photometry."
Comments P.1 right column top paragraph: "Estimates of actin filament concentrations in lamellipodia range from 700 µM, based on filament counts from electron microscopy [ref 18 BNID 112789] to 1600 µM, derived from the comparison of the phalloidin label intensities of single filaments and lamellipodia of fixed cells [primary source 19 BNID 112789]. The latter authors concluded that the G-actin concentration at the lamellipodium tip was in the range of 8 µM, based on in vitro rate constants for polymerization [primary source 19]."
Entered by Uri M
ID 112790