Retrograde flow of lamellipodium of keratocytes

Range ~5 µm/min
Organism Molly fish Poecillia sphenops
Reference Sens P, Plastino J. Membrane tension and cytoskeleton organization in cell motility. J Phys Condens Matter. 2015 Jul 15 27(27):273103. doi: 10.1088/0953-8984/27/27/273103. p.9 right column top paragraphPubMed ID26061624
Primary Source [67] Jurado C, Haserick J R and Lee J 2005 Slipping or gripping? Fluorescent speckle microscopy in fish keratocytes reveals two different mechanisms for generating a retrograde flow of actin Mol. Biol. Cell 16 507–18 [70] Doyle A, Marganski W and Lee J 2004 Calcium transients induce spatially coordinated increases in traction force during the movement of fish keratocytes J. Cell Sci. 117 2203–14PubMed ID15548591, 15126622
Method Primary source [67] abstract: "To investigate the relationship between retrograde flow and traction force generation, [investigators] have transfected keratocytes with GFP-actin and used fluorescent speckle microscopy, to observe speckle flow." Primary source [70] abstract: "Although it is likely that retraction involves a calcium-dependent increase in cytoskeletal contractility, it is not known how the timing, magnitude and localization of contractile forces are organized during retraction. [Investigators] have addressed this question using a new gelatin traction force assay in combination with calcium imaging to determine what changes in cytoskeletal force production accompany calcium-induced retraction."
Comments P.9 right column top paragraph: "Traction force measurements show that the lamellipodium of keratocytes exerts traction stress, αvr, on the order of 100 Pa and have retrograde flow on the order of about 5μm/min [primary sources], giving α ≈ 10^3 Pa×sμm^−1."
Entered by Uri M
ID 112514