Diffusion coefficients for lipid and membrane proteins in GUVs (giant unilamellar vesicles)

Range Table - link 10^-8 cm^2/s
Organism Unspecified
Reference Doeven MK et al., Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles. Biophys J. 2005 Feb88(2):1134-42. p.1139 table 1PubMed ID15574707
Method P.1138 right column bottom paragraph:"The mobility of OppA, MscL, and LacS in GUVs was determined by FCS [fluorescence correlation spectroscopy] measurements. Confocal images were made of proteo-GUVs containing fluorescent labeled protein, and the focal volume was focused on the pole of the GUVs. Representative autocorrelation curves for each of the three proteins studied are shown in Fig. 5 D the experimental data could be fitted reasonably well with a one-component two-dimensional diffusion model. Occasionally the membranes appeared to move or fluctuate during the autocorrelation measurements, which could be observed as a decay or systematic deviation in the count rate. Autocorrelation curves affected by these membrane undulations were not taken into account when analyzing the data."
Comments P.1138 right column bottom paragraph:"Undulations seemingly appeared more frequently when measuring autocorrelation curves with very large GUVs (30–100 μm) and less when the GUVs were somewhat smaller (<30 μm). The measured diffusion coefficients are summarized in Table 1. Lipid-anchored oligopeptide-binding protein OppA diffused with the same speed as the fluorescent lipid DiO. The mobility of the integral membrane proteins MscL and LacS was lower, with LacS being the slowest. The lateral mobility of the integral membrane proteins was ∼2–3-fold lower than the mobility of DiO." See notes beneath table
Entered by Uri M
ID 112264