Death rate of lexA3 mutant cells

Value 2.7 %/cell/generation
Organism Bacteria Escherichia coli
Reference Wang P et al., Robust growth of Escherichia coli. Curr Biol. 2010 Jun 22 20(12):1099-103. doi: 10.1016/j.cub.2010.04.045. p.1100 right column bottom paragraph p.1102 figure 4PubMed ID20537537
Method Abstract:"[Investigators] studied the long-term growth and division patterns of Escherichia coli cells by employing a microfluidic device designed to follow steady-state growth and division of a large number of cells at a defined reproductive age."
Comments p.1100 right column bottom paragraph:"Because filamentation is a hallmark of the SOS response in bacteria, [investigators] asked how its suppression would affect [their] observation of filamentation. For this purpose [they] constructed an MG1655 derivative carrying a lexA allele, lexA3, whose protein product constitutively represses SOS gene expression even under conditions of DNA damage. Although the lexA3 mutant behaved virtually the same as MG1655 in terms of a constant growth rate, its filamentation rate, which was constant at approximately 1%, was significantly reduced, as expected. Note that B/r lacks sulA, a key SOS gene that inhibits cell division during the SOS response, and also shows a similar low filamentatin rate (Figure S3). A more important difference between lexA3 and MG1655 is that, with a constant death rate of 2.7% per cell per generation, the population of the lexA3 mutant cells decayed exponentially (Figure 4)."
Entered by Uri M
ID 112058