Concentration gradient at which functional chemotaxis is observed away from capillary tube

Value 0.02 µM/µm
Organism Bacteria Escherichia coli
Reference Sourjik V, Berg HC. Receptor sensitivity in bacterial chemotaxis. Proc Natl Acad Sci U S A. 2002 Jan 8 99(1):123-7. p.126 left column bottom paragraphPubMed ID11742065
Method "[Investigators] used fluorescence resonance energy transfer (FRET) to measure intracellular changes in phosphorylation-dependent interactions of CheY upon chemotactic stimulation."
Comments "The changes in receptor occupancy encountered by bacteria swimming in spatial gradients (e.g., near the mouth of a capillary tube in the capillary assay) are very small. For example, in the tracking experiments (ref 1 PMID 4563019), cells about 0.6 mm from the tip of a capillary tube containing 1 mM aspartate moved in a gradient of steepness 0.02 µM/µm at a mean concentration of about 8µM."
Entered by Uri M
ID 111492