Estimated ATP stoichiometry parameters

Range Table - link
Organism Microbes
Reference Taymaz-Nikerel H, Borujeni AE, Verheijen PJ, Heijnen JJ, van Gulik WM. Genome-derived minimal metabolic models for Escherichia coli MG1655 with estimated in vivo respiratory ATP stoichiometry. Biotechnol Bioeng. 2010 Oct 1 107(2):369-81. doi: 10.1002/bit.22802. p.377 table VIIIPubMed ID20506321
Primary Source Van Gulik WM, Heijnen JJ. 1995. A metabolic network stoichiometry analysis of microbial growth and product formation. Biotechnol Bioeng 48(6):681–698. & Van Gulik WM, Antoniewicz MR, deLaat WTAM, L VJ, Heijnen JJ. 2001. Energetics of growth and penicillin production in a high-producing strain of Penicillium chrysogenum. Biotechnol Bioeng 72(2):185–193.PubMed ID18623538, 11114656
Method Abstract:"The unknown ATP stoichiometry parameters of the constructed E. coli network were estimated from experimental data of eight different aerobic chemostat experiments carried out with E. coli MG1655, grown at different dilution rates (0.025, 0.05, 0.1, and 0.3 h(-1)) and on different carbon substrates (glucose, glycerol, and acetate)."
Comments "The estimated ATP stoichiometry parameters are shown in Table VIII. It can be seen from this table that the estimated d is significantly lower than the maximum theoretical value (2.0). Although under high oxygen levels (as was the case in [investigators’] chemostat cultures) the predominant terminal oxidase is of the bo-type with an H?+/e- ratio of 2, under these conditions also the NDH2 dehydrogenase, which does not contribute to the pmf, is known to be expressed (see Theoretical Aspects Section). This implies that the expected d under these conditions would be between 1.0 and 2.0, which corresponds well with the estimated value of 1.49." "It is also worthwhile to compare [investigators’] in vivo E. coli results with those for S. cerevisiae (Van Gulik and Heijnen, 1995) and P. chrysogenum (Van Gulik et al., 2001) which are of the same order of magnitude (Table VIII). [Investigators’] estimation of the d of E. coli lies between the values for S. cerevisiae and P. chrysogenum. The growth related maintenance coefficient for E. coli was similar as for P. chrysogenum whereas the growth independent maintenance coefficient was two times higher for E. coli."
Entered by Uri M
ID 111461