||Lee H, Popodi E, Tang H, Foster PL. Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing. Proc Natl Acad Sci U S A. 2012 Oct 9 109(41):E2774-83. doi: 10.1073/pnas.1210309109. p.E2774 right column top paragraphPubMed ID22991466
||Lynch M, Walsh JB (1998) Genetics and Analysis of Quantitative Traits (Sinauer Associates., Inc, Sunderland, MA).
||"The mutation-accumulation (MA) strategy combined with whole-genome sequencing overcomes many of these limitations. The MA
protocol is designed to allow mutations to occur in a neutral manner, devoid of selective pressure (ref 5). The general strategy is to establish a number of clonal populations from a founder individual
and then to take each population through repeated single-individual
bottlenecks for thousands of generations. Because the effective
population size of each line is one, genetic drift prevents selection
from eliminating all but the most deleterious mutations, which
typically are less than 1% of mutations (primary source)."