Estimated maximal velocity of ADP-utilizing enzymes, assuming calculated free ADP values for cytoplasm

Range Table - link
Organism Mammals
Reference Veech RL, Lawson JW, Cornell NW, Krebs HA. Cytosolic phosphorylation potential. J Biol Chem. 1979 Jul 25 254(14):6538-47. p.6546 table VIPubMed ID36399
Method See note above table
Comments "Calculation of metabolite concentrations used in table VI-Since the total concentration of glyceraldehyde-3-P in tissue is very low and is similar in order of magnitude to the concentration of the enzymes for which it is a substrate, there is considerable uncertainty about the amount of bound and free glyceraldehyde-3-P (23). Therefore, the concentration of free cytosolic glyceraldehyde-3-P has been estimated using the measured dihydroxyacetone-P, which has been shown to represent the free cytosolic concentration, and the equilibrium constant of triose phosphate isomerase [EC] was determined in vitro (23)."
Entered by Uri M
ID 110947