HeLa cell doubling time grown in SILAC medium

Value 24.67 hours
Organism Human Homo sapiens
Reference Boisvert FM, Ahmad Y, Gierlinski M, Charrière F, Lamont D, Scott M, Barton G, Lamond AI. A quantitative spatial proteomics analysis of proteome turnover in human cells. Mol Cell Proteomics. 2012 Mar11(3):M111.011429. doi: 10.1074/mcp.M111.011429. p.6 right column 3rd paragraphPubMed ID21937730
Method "[Researchers] combine an enhanced pulse SILAC (stable isotope labeling with amino acids in cell culture) approach with spatial proteomics to perform a system-wide analysis of protein turnover in cultured human cells."
Comments "[~20 hours, average protein turnover rate, BNID 109937] is close to the cell doubling time under the growth conditions used which [researchers] measured to be 24.67 h for HeLa cells growing in the SILAC medium, consistent with approximate doubling of the protein content as the cell divides (Fig. 3, red line)."
Entered by Uri M
ID 109938