Total RNA content in cell

Range ~10 pg/cell
Organism Mammals
Reference Tang F, Lao K, Surani MA. Development and applications of single-cell transcriptome analysis. Nat Methods. 2011 Apr8(4 Suppl):S6-11. doi: 10.1038/nmeth.1557. p.3 bottom paragraphPubMed ID21451510
Primary Source Kurimoto K, et al. An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis. Nucleic Acids Res. 2006 34:e42. AND Livesey FJ. Strategies for microarray analysis of limiting amounts of RNA. Brief. Funct. Genomic Proteomic. 2003 2:31–36. AND Kawasaki ES. Microarrays and the gene expression profile of a single cell. Ann. N Y Acad. Sci. 2004 1020:92–100.PubMed ID16547197, 15239941, 15208186
Comments "After reverse transcription and tailing steps, the single cell cDNAs can be amplified. One representative individual mammalian cell contains about 10 pg of total RNAs, and about 0.1 pg of mRNAs, which usually needs to be amplified around ten million folds to match the requirement for a standard microarray analysis. Either PCR or IVT can be used for amplification (primary sources)."
Entered by Uri M
ID 109740