Protein content in liver mitochondrial matrix

Value 47.3 %
Organism Rat Rattus norvegicus
Reference Hoppel CL, Tomec RJ. Carnitine palmityltransferase. Location of two enzymatic activities in rat liver mitochondria. J Biol Chem. 1972 Feb 10 247(3):832-41. p.835 table IIPubMed ID5058225
Method "Protein Determination-The biuret method (Gornall, A. G., Bardawill, C. J., and David, M.M. (1949) J. Biol. Chem., 177, 751) was used following: precipitation with perchloric acid (intracellular distribution studies) or solubilization with deoxycholate (mitochondrial fractionation)."
Comments "The protein content of the matrix is based on the ratio of the specific activity of a matrix enzyme of whole mitochondria compared to its specific activity in isolated matrix fraction."
Entered by Uri M
ID 108993