| Value |
20
Sec
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Lieberman BA, Nordeen SK. DNA intersegment transfer, how steroid receptors search for a target site. J Biol Chem. 1997 Jan 10 272(2):1061-8. p.1064 right column 3rd paragraphPubMed ID8995403
|
| Method |
A half-site
oligonucleotide was used to measure the equilibrium binding
constant K1 for GRdbd (glucocorticoid receptor
DNA binding domain protein fragment) monomer binding to DNA. |
| Comments |
See BNID 106483, 106484, 106485. Nonspecific DNA
increased the monomer dissociation rate to the limit of researchers'
systems measurement (t1/2˜20s). Conc. of 22µg/ml is equivalent to 1×10^-6Mbp |
| Entered by |
Uri M |
| ID |
106486 |