Average mRNA halflife in embryo

Value 12.9 hours
Organism Sea urchin Strongylocentrotus purpuratus
Reference Howard-Ashby M, Materna SC, Brown CT, Chen L, Cameron RA, Davidson EH. Identification and characterization of homeobox transcription factor genes in Strongylocentrotus purpuratus, and their expression in embryonic development. Dev Biol. 2006 Dec 1 300(1):74-89 p. 82 table 2 Table - link PubMed ID17055477
Method QPCR was used to determine the expression profile of each identified transcription factor from unfertilized egg to 48 h. mRNA was isolated from egg, 6, 12, 18, 24, 36 and 48 h embryos with the Sigma GenElute Mammalian Total RNA Miniprep Kit, per the manufacturers instructions.
Comments As expected, there was much variation among the half-lives of different transcripts. Sp-nk7 had the shortest half-life, at 3.30 h, while Sp-pax2/5/8 had the longest, at 31.4 h. The average half-life of any message was 12.9 h, about twice the 5.7-h average half-life for total polysomal RNA transcript in the S. purpuratus blastula–gastrula embryo (Davidson, 1986). The rate of synthesis of different mRNAs was likewise quite variable, ranging from 36 molecules/h (Sp-lhx2) to 550 molecules/h (Sp-irxA). The average rate of synthesis was about 150 molecules/h per embryo.
Entered by Uri M
ID 105630