Number of of EnvZ molecules per cell

Value 102 Copies/cell Range: 80-128 Copies/cell
Organism Bacteria Escherichia coli
Reference Cai SJ, Inouye M. EnvZ-OmpR interaction and osmoregulation in Escherichia coli. J Biol Chem. 2002 Jul 5 277(27):24155-61 p.24158 Table IVPubMed ID11973328
Method Although the envZ gene is in the same operon as the ompR gene (see BNID 105109), their expression levels are known to be significantly different. Previously the cellular level of EnvZ was estimated to be 10 molecules per cell (5). Researchers used the membrane fraction rather than the whole cell lysate for Western blot analysis to determine the cellular EnvZ levels. Densitometric analysis of EnvZc standards was plotted as shown in Fig. 2B, and the amount of EnvZ was calculated from the standard curve.
Comments Values are average and range for MC4100 grown in L-broth, table IV. The wild-type strain MC4100 grown in L-broth medium in mid-log phase was estimated to contain about 102 EnvZ monomers per cell (or 51 dimers per cell), whereas 63 and 113 EnvZ monomers per cell (or 32 and 57 dimers per cell) are present when cells were grown in NB (nutrient broth, low osmolarity, table VI) or in NB + 20% sucrose (high osmolarity, table V), respectively. See BNID 105109,105111
Entered by Uri M
ID 105110