Dissociation constant of GDP in complex with Ef-TU

Value 12 nM Range: ±1.6 nM
Organism Bacteria Escherichia coli
Reference Navratil T, Spremulli LL. Effects of mutagenesis of Gln97 in the switch II region of Escherichia coli elongation factor Tu on its interaction with guanine nucleotides, elongation factor Ts, and aminoacyl-tRNA.Biochemistry. 2003 Nov 25 42(46):13587-95PubMed ID14622005
Method Two independent preparations of wild-type and Q97P variant EFTu proteins purified separately were characterized. The error analysis was performed according to ref 25.
Comments Total [EF-Tu]was(6.9 nM), total [GDP]was (15 nM) and [Tu-GDP] was(3.4 nM)
Entered by Uri M
ID 104114