Osmotic coefficients and volumes of bound and free water in the cytoplasm of E. coli grown at low and moderate osmolarity

Range Table - link
Organism Bacteria Escherichia coli
Reference Cayley S, Lewis BA, Guttman HJ, Record MT Jr. Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity. Implications for protein-DNA interactions in vivo. J Mol Biol. 1991 Nov 20 222(2):281-300 p.289 table 3PubMed ID1960728
Method In vivo osmotic coefficients were determined from the variation of cytoplasmic volume with external osmolarity in plasmolysis titrations of non-growing cells. The values obtained (f = 0.54(±0.06) for cells grown at 0.10 OSM and f = 0.71(±0.11) at 0.28 OSM indicate a high degree of non-ideality of intracellular ions arising from coulombic interactions between K+ and cytoplasmic polyanions.
Comments An osmotic coefficient f is a quantity which characterises the deviation of a solvent from ideal behaviour, referenced to Raoult's law.
Entered by Uri M
ID 104051