Diffusion coefficient of Insulin Growth Factor-I through fibrin gel

Value 159 µm^2/sec Range: ±16 µm^2/sec
Organism Generic
Reference Nauman JV, Campbell PG, Lanni F, Anderson JL. Diffusion of insulin-like growth factor-I and ribonuclease through fibrin gels. Biophys J. 2007 Jun 15 92(12):4444-50.PubMed ID17400703
Method Each experimental run was initiated by adding one or both labeled proteins in gel buffer at the gel-solution interface using a flexible needle to create a step function of concentration. Initial protein concentration was set at 800 nM for IGF-I. All experiments were run at 24°C, and the results corrected to 25°C using the relation D?/T = constant, where ? is the viscosity of water and T is the absolute temperature. Table 1 shows the values of D obtained by fitting Eq. 4 to the data from each experiment for the two labeled proteins, combining data from both image fields at five time points each.
Comments Experiment goal was to simulate diffusion of protein in Extra-Cellular Matrix, ECM.
Entered by Uri M
ID 104031