Plastocyanin concentration at onset of stationary phase

Value 35 µmole/mole chlorophyll Range: ±10 µmole/mole chlorophyll
Organism Chlamodymonas mundana
Reference Wood PM. Interchangeable copper and iron proteins in algal photosynthesis. Studies on plastocyanin and cytochrome c-552 in Chlamydomonas. Eur J Biochem. 1978 Jun 187(1):9-19 Table - link PubMed ID208838
Method For chlorophyll assays the material was added to acetone and hand homogenised. For complete ex1 raction of soluble proteins (plastocyanin and cytochromes c-552 and c-550) the cells were spun down, washed in 10 mM phosphate pH 7, and frozen at - 80 degrees C in 10 mM phosphate, pH 7 plus 0.4 M NaCl.
Comments On aerated medium, with copper added. See table for details
Entered by Uri M
ID 104026