Constant for binding of repressor to O3 locus of lac operator

Value 110 nM
Organism Bacteria Escherichia coli
Reference Bintu L, Buchler NE, Garcia HG, Gerland U, Hwa T, Kondev J, Kuhlman T, Phillips R. Transcriptional regulation by the numbers: applications. Curr Opin Genet Dev. 2005 Apr15(2):125-35 fig 6bPubMed ID15797195
Primary Source Oehler S, Amouyal M, Kolkhof P, von Wilcken-Bergmann B, Müller-Hill B. Quality and position of the three lac operators of E. coli define efficiency of repression. EMBO J. 1994 Jul 1513(14):3348-55PubMed ID8045263
Method The expected fold-change is given by Case 1 (of Table 1 in companion article, pubmed id 15797194) , with [R] = [LacI4], KR = Km and only one unknown parameter, Km, characterizing the effective dissociation constant of the operator Om. Here, it is possible to compute Km directly from the experimental data of Oehler et al. (primary ref)
Comments This mathematical model (see companion article pubmed id 15797194) obtained value differs significantly from the experimentally obtained in vitro value of 0.016-1 nM
Entered by Uri M
ID 103597