Size of typical tether in Tethered Particle Motion method

Value 1000 bp
Organism Generic
Reference Garcia HG, Grayson P, Han L, Inamdar M, Kondev J, Nelson PC, Phillips R, Widom J, Wiggins PA. Biological consequences of tightly bent DNA: the other life of a macromolecular celebrity. Biopolymers. 2007 Feb 585(2):115-30.PubMed ID17103419
Method TPM-Individual molecules of a specifically biotinated (RNA in this case) polymerase derivative were tagged an avidin-coated polystyrene beads enzyme movement along a surface-linked DNA molecule was monitored by observing changes in bead Brownian motion by light microscopy.
Entered by Uri M
ID 103168