Decrease of repression of lac transcription in absence of one 'pseudo operator'

Range 2-3 Fold
Organism Bacteria Escherichia coli
Reference Oehler S, Eismann ER, Krämer H, Müller-Hill B,The three operators of the lac operon cooperate in repression,EMBO J. 1990 Apr9(4):973-9PubMed ID2182324
Method testing the effect of systematic destruction of all three lac operators of the chromosomal lac operon of Escherichia coli on repression by Lac repressor
Comments With all three operators, O1,O2,O3 expression of beta-galactosidase is repressed 1300 Fold. destruction of 'pseudo operators' O2 or O3 results in 2-3 fold decrease in repression relative to 1300
Entered by Uri M
ID 102089