Density of NPCs (Nuclear Pore Complexes) during early mitosis

Value 11.4 NPC/µm^2 Range: ±1.55 NPC/µm^2
Organism Budding yeast Saccharomyces cerevisiae
Reference Winey M, Yarar D, Giddings TH Jr, Mastronarde DN. Nuclear pore complex number and distribution throughout the Saccharomyces cerevisiae cell cycle by three-dimensional reconstruction from electron micrographs of nuclear envelopes. Mol Biol Cell. 1997 Nov8(11):2119-32. p. 2124 table 1PubMed ID9362057
Method P.2122 left column 2nd paragraph: "[Researchers] created 3D reconstructions of entire nuclei from electron micrographs of serial thin sections to determine the number, surface density, and distribution of NPCs [Nuclear Pore Complexes] throughout the cell cycle of the budding yeast S. cerevisiae."
Comments P.2122 right column bottom paragraph to p.2124 left column: "The IMOD program [a tool for analyzing and viewing three-dimensional biological image data] was used to extract various parameters from the 32 models of yeast nuclei. These values include the number of NPCs per nucleus, as well as the volume and surface area of each nucleus (Table 1). Surface area was determined from a mesh of triangles over the surface of the nuclear models (Figure 2, see MATERIALS AND METHODS). By using the surface area and NPC values for each nucleus, an average NPC density (NPC/μm^2 of nuclear envelope) was derived (Table 1). The number of NPCs observed in models of individual nuclei ranged from 65 in a G1 cell (Table 1, model 1) to 182 NPCs in a late anaphase cell (Table 1, model 32). The surface area of the nuclei ranged from 5.5 μm^2 in a G1 cell to 16.9 μm^2 in a late anaphase cell (Table 1, models 5 and 30, respectively). The volume of the nuclei ranged from 1.3 μm^3 in a S-phase cell to 4.0 μm^3 in a mitotic cell (Table 1, models 12 and 24, respectively). The trend toward increasing numbers in later stages of the cell cycle is reversed for average NPC density per μm^2 of nuclear envelope, which ranged from 8.2 NPCs/μm^2 in a late anaphase cell to 18 NPCs/μm^2 in a S-phase cell (Table 1, models 27 and 15, respectively)."
Entered by Ben Marks
ID 101348