| Value |
75
Copies/µm^2
Range: ±20 Copies/µm^2
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Lelkes PI, Klein L, Marikovsky Y, Eisenbach M. Liposome-mediated transfer of macromolecules into flagellated cell envelopes from bacteria. Biochemistry. 1984 Jan 31 23(3):563-8.PubMed ID6367823
|
| Method |
The transfer of liposome-encapsulated ferritin was vizualized
in ultrathin sections of cell envelopes treated with liposomes,
for 30 min. The incubation mixture was fixed in 2.5% formaldehyde
and further processed as described (Eisenbach &
Adler, 1981). Embedding was in Epon 812. Thin sections
(400-500 Å thick) were cut with a Sorval MT2 ultramicrotome
and collected on formvar-coated grids. All micrographs
were taken in a JEOL JEM-7 electron microscope, operated
at 80 kV. |
| Comments |
A random
count in ca. 200 cell envelopes indicates that there are some
75±20 ferritin molecules/µm^2. |
| Entered by |
Ron Milo - Admin |
| ID |
101064 |