Cost of biosynthesis of methionine (most expensive amino acid) from glucose

Value 18 mol ATP
Organism Bacteria Escherichia coli
Reference Kaleta C, Schäuble S, Rinas U, Schuster S. Metabolic costs of amino acid and protein production in Escherichia coli. Biotechnol J. 2013 Sep8(9):1105-14. doi: 10.1002/biot.201200267 p.1108 table 1, p.1109 left column top paragraph and p.1113 right column 2nd paragraphPubMed ID23744758
Primary Source [7] Park, J. H., Lee, S. Y., Metabolic pathways and fermentative production of L‐aspartate family amino acids. Biotechnol. J. 2010, 5, 560–577 DOI: 10.1002/biot.201000032 [31] Krömer, J. O., Wittmann, C., Schroder, H., Heinzle, E., Metabolic pathway analysis for rational design of L‐methionine production by Escherichia coli and Corynebacterium glutamicum. Metab. Eng. 2006, 8, 353–369 DOI: 10.1016/j.ymben.2006.02.001 [35] Ikeda, M., Amino acid production processes. Adv. Biochem. Eng. Biotechnol. 2003, 79, 1–35PubMed ID20518059, 16621639, 12523387
Comments P.1108 note 'b' beneath table 1: "ATP cons. indicates the number of ATP consumed according to the manual calculations for each carbon source as indicated on the top of the rows (assuming 1 NADH = 1 NADPH = 2 FADH = 2 ATP)." P.1109 left column top paragraph: "The most expensive amino acid is methionine, which consumes 18 mol of ATP, which arises primarily from incorporating sulfur." P.1113 right column 2nd paragraph: "Assuming canonical routes for the production of amino acid precursors, methionine is the most expensive amino acid. Its biosynthesis from glucose costs 18 mol of ATP, which is of particular importance for the design of production processes and strains over-producing this amino acid, as it is one of the most important commercial amino acids [primary sources]."
Entered by Uri M
ID 114973