Resting Membrane Potential

Range -51±2.4 mV
Organism HeLa cell line
Reference Stein MA et al., Enteropathogenic Escherichia coli markedly decreases the resting membrane potential of Caco-2 and HeLa human epithelial cells. Infect Immun. 1996 Nov64(11):4820-5 p.4822 left column 2nd paragraphPubMed ID8890244
Method Abstract: "In this study [investigators] used the whole-cell, current clamp variant of the patch clamp technique to demonstrate that EPEC infection of HeLa and Caco-2 human epithelial cells reduces cell resting membrane potential."
Comments P.4822 left column 2nd paragraph: "EPEC [Enteropathogenic Escherichia coli] depolarizes HeLa and Caco-2 [human epithelial cells] cell membranes. HeLa cells which exhibited at least one attached wild-type EPEC microcolony had a significantly lower mean RMP [resting membrane potential] than was observed in cells incubated in MEM [minimal essential medium] alone (Fig. 1). The mean RMP for untreated HeLa cells was -51±2.4 mV (n=23 cells), and the mean RMP for EPEC-treated cells was -25±1.2 mV (n=13 cells, P<0.001, by analysis of variance [ANOVA]). Incubation of HeLa cells with the supernatant from preinduced EPEC cultures failed to elicit a drop in RMP relative to control values. The mean RMP for 10 HeLa cells treated with EPEC supernatant was -48±3.0 mV (Fig. 1). This indicates that the EPEC-mediated drop in RMP was not due to the alteration of medium composition by bacterial growth or the elaboration of bacterial components into the medium. "
Entered by drothbard
ID 114263