Comparison of yield and methodologies employed in the native producers of coenzyme Q10

Range Table - link mg/gDCW
Organism Microbes
Reference Lee SQ, Tan TS, Kawamukai M, Chen ES. Cellular factories for coenzyme Q10 production. Microb Cell Fact. 2017 Mar 2 16(1):39. doi: 10.1186/s12934-017-0646-4. p.6 table 1PubMed ID28253886
Primary Source See refs beneath table
Method See "Methodologies" column
Comments P.6 left column 3rd paragraph: "Schizosaccharomyces pombe (fission yeast) is a well-studied model organism with similar molecular pathway makeup and genetic mechanisms as those in humans [refs 89, 90]. However, little effort has been made to develop S. pombe into a suitable framework for high-value compound production [ref 91], and so efforts to increase CoQ10 in S. pombe have thus far been limited. In one study, genes encoding enzymes directly involved in CoQ10 biosynthesis (dps1 +–dlp1 +, ppt1 +, and coq3 +–coq9 +) and HMGR [HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) reductase][ref 83] were overexpressed. However, only overexpression of HMGR—and not the CoQ10 biosynthesis genes—led to a prominent 2.7-fold increase in CoQ10 yield (Table 1). It was posited that the lack of effect from the biosynthetic genes was because these enzymes are not rate-limiting."
Entered by Uri M
ID 113504