Number of Rkr1 proteins [involved in ubiquitin-mediated degradation of non-stop proteins and translationally stalled ER membrane proteins]

Value 200 proteins/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Duttler S, Pechmann S, Frydman J. Principles of cotranslational ubiquitination and quality control at the ribosome. Mol Cell. 2013 May 9 50(3):379-93. doi: 10.1016/j.molcel.2013.03.010. p.388 right column bottom paragraphPubMed ID23583075
Comments P.388 right column bottom paragraph: "[Investigators'] analysis of RKR1 deletion strains suggests that only a relatively small fraction of ubiquitinated nascent chains arises from stalled or NS-mRNA-derived products (Bengtson and Joazeiro, 2010). This most likely reflects the robust nature of mRNA quality control (Doma and Parker, 2007), which rapidly eliminates aberrant mRNAs in an initial round of translation. Consistent with these findings, there are only 200 molecules of Rkr1 compared to 200,000 ribosomes per yeast cell (Ghaemmaghami et al., 2003 von der Haar, 2008 BNID 100267)."
Entered by Uri M
ID 113363