Half-time for repair of dehydratase clusters

Range ~5 min
Organism Bacteria Escherichia coli
Reference Imlay JA. The molecular mechanisms and physiological consequences of oxidative stress: lessons from a model bacterium. Nat Rev Microbiol. 2013 Jul11(7):443-54. doi: 10.1038/nrmicro3032. p.446 Box 2 top paragraphPubMed ID23712352
Primary Source [140] Gardner, P. R. & Fridovich, I. Inactivation-reactivation of aconitase in Escherichia coli. A sensitive measure of superoxide radical. J. Biol. Chem. 267, 8757–8763 (1992).PubMed ID1315737
Method Primary source abstract: "The rapid inactivation of aconitase by O2-, previously seen to occur in vitro, was explored in vivo."
Comments P.446 Box 2 top paragraph: "Because the half-time for repair of dehydratase clusters is about 5 minutes [primary source], at any moment during aerobic growth a significant minority of the enzyme population is inactive. Any additional H2O2 stress will exacerbate the situation."
Entered by Uri M
ID 112946