Fluorescence intensity statistics for individual components as determined from MCR [multivariate curve resolution] analysis of hyperspectral images

Range Table - link
Organism Cyanobacteria Synechocystis PCC 6803
Reference Vermaas WF et al., In vivo hyperspectral confocal fluorescence imaging to determine pigment localization and distribution in cyanobacterial cells. Proc Natl Acad Sci U S A. 2008 Mar 11 105(10):4050-5. doi: 10.1073/pnas.0708090105. Supporting Information table 1PubMed ID18316743
Method Note beneath table:"Percentages reported were calculated by determining the mean fluorescence intensity of each component at its emission maximum from all of the cells in an image of a strain and dividing that value by the sum of the mean values for all components for that strain. All percentages except those indicated by an asterisk are statistically supported. The value in the last column indicates the percentage of total signal relative to the wild-type Synechocystis grown in light. Note: The carotenoid mean intensities were calculated by using the baseline-corrected intensity at the most intense Raman peak, rather than at the emission maximum as done for the fluorescence emission components."
Comments p.4052 right column 3rd paragraph:"In Synechocystis, Chl with long-wavelength fluorescence emission is associated primarily with PS I (ref 37), and the distribution of the Chl-698 component is more centrally located within the cell compared with the Chl-685 component (Figs. 2 and 3). To further explore this apparent discrepancy, a PS I-less mutant (ref 31) was investigated (Fig. 3). As shown in Fig. 3 and SI Table 1, the Chl-698 component in the PS I-less strain was so low as to be indistinguishable from the noise for most voxels, strengthening both the assignment of the Chl-698 component to PS I-associated Chl and the confidence in the quality of the resolution of the pure-component spectra."
Entered by Uri M
ID 111792