| Method |
Value was extracted visually from fig.S1A "[Investigators] developed a model system that allows [them] to monitor
metabolic differences between proliferating and quiescent cells.
Primary dermal fibroblasts were expanded and analyzed while
actively proliferating, after 1 wk of growth to confluence (contact-inhibited
for 7 d [CI7]), after 2 wk of confluence (contact-inhibited
for 14 d [CI14]), or after 2 wk of confluence with serum
concentrations decreased for the final week from 10% to 0.1%
(contact-inhibited for 14 d and serum-starved for 7 d [CI14SS7]). Alternatively, fibroblasts were plated sparsely so that they did not
touch each other and induced into quiescence by serum starvation
and monitored after 4 d (serum-starved for 4 d [SS4]) or 7 d
(serum-starved for 7 d [SS7])." "Rates of glucose consumption, lactate
excretion, glutamine consumption, and glutamate excretion were
monitored in proliferating, CI7, CI14, CI14SS7, SS4, SS7,
proliferating low glucose/low glutamine, and CI14 low glucose/
low glutamine fibroblasts using the YSI 7100 bioanalyzer. Levels
were normalized for the amount of cellular protein present during
the conditioning time. Error bars indicate standard error." |