Summary of morphometric data for enterocytes

Range Table - link
Organism Human Homo sapiens
Reference Crowe PT, MarshMN(1993) Morphometric analysis of small intestinal mucosa. IV. Determining cell volumes. Virchows Arch A Pathol Anat Histopathol 422: 459–466 p.462 table 2PubMed ID8333150
Method "The subjects consisted of ten disease-control patients (7 male/4 female age range 23-48 years) in whom a jejunal biopsy was performed as part of their diagnostic work-up, but in whom gluten sensitivity was excluded by the presence of a normal biopsy. There were also 11 healthy volunteers (5 male and 6 female all under 30 years of age) recruited from the medical and paramedical staff of this hospital. These 21 control mucosae were compared with jejunal mucosae obtained from 20 patients (8 females, aged 2~58 years and 12 males, aged 25-72 years) with untreated gluten sensitivity." (Abstract:) "Four procedures were employed, of which two were geometrical, based on cylindrical or truncated conoid models. The third method evolved from the proportionality of area to volume, and required determination of cellular and nuclear profile areas, and an estimation of nuclear volume based on models conforming to (1) a prolate spheroid, or (2) a cylinder with hemispherical caps. This procedure appeared to underestimate enterocyte volumes and failed to reveal volume differences between controls and gluten-sensitive individuals. Finally, a fourth method was devised, based on traditional intraepithelial profile counts per hundred enterocyte nuclei, calculation of surface epithelial volume and of the absolute number of lymphocytes contained therein."
Comments "Since many of these values were previously unknown, the data were first analysed for normality of distribution. For most, the degree of kurtosis was tolerable and parametric tests were employed, the main exceptions being volume data both for enterocytes and enterocyte nuclei which were therefore transformed logarithmically. Since IEL density counts are non-normally distributed, all values and calculations pertaining to such data (Table 2) were also log-transformed."
Entered by Uri M
ID 111219