Michaelis–Menten constant (Km) of proteorhodopsin

Value 1000 photons/sec/nm^2
Organism Bacteria Escherichia coli
Reference Walter JM, Greenfield D, Bustamante C, Liphardt J. Light-powering Escherichia coli with proteorhodopsin. Proc Natl Acad Sci U S A. 2007 Feb 13 104(7):2408-12. DOI: 10.1073/pnas.0611035104 abstractPubMed ID17277079
Method Abstract: "By measuring the pmf [proton motive force] of individual illuminated cells, [researchers] quantify the coupling between light-driven and respiratory proton currents, estimate the Michaelis–Menten constant (Km) of PR [Proteorhodopsin] (10^3 photons per second/nm^2), and show that light-driven pumping by PR can fully replace respiration as a cellular energy source in some environmental conditions."
Entered by Uri M
ID 111064