Number of copies of pyruvate decarboxylase

Value 23400 molecules/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Carroll KM, Simpson DM, Eyers CE, Knight CG, Brownridge P, Dunn WB, Winder CL, Lanthaler K, Pir P, Malys N, Kell DB, Oliver SG, Gaskell SJ, Beynon RJ. Absolute quantification of the glycolytic pathway in yeast: deployment of a complete QconCAT approach. Mol Cell Proteomics. 2011 Dec10(12):M111.007633. doi: 10.1074/mcp.M111.007633. p.10 left column 2nd paragraphPubMed ID21931151
Method "[Researchers] developed the QconCAT approach for multiplexed absolute quantification (refs 6, 7). In brief, synthetic genes, optimized for heterologous expression in Escherichia coli, encode a single open reading frame that is a concatenation of tryptic peptides, each of which acts as an internal standard (a Q-peptide) for a defined protein. Each analyte protein is represented by at least one, but more preferably two (or more), Q-peptides."
Comments "Of the two peptides selected for Pdc6p, IATTGEWDALTTDSEFQK and LPVFDAPESLIK, the former was detected using an SRM [selected reaction monitoring] approach with 23,400 molecules/cell obtained corresponding to ~800 amol on column for the most abundant extract."
Entered by Uri M
ID 110557