| Value |
123000
molecules/cell
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Carroll KM, Simpson DM, Eyers CE, Knight CG, Brownridge P, Dunn WB, Winder CL, Lanthaler K, Pir P, Malys N, Kell DB, Oliver SG, Gaskell SJ, Beynon RJ. Absolute quantification of the glycolytic pathway in yeast: deployment of a complete QconCAT approach. Mol Cell Proteomics. 2011 Dec10(12):M111.007633. doi: 10.1074/mcp.M111.007633. p.7 right column bottom paragraphPubMed ID21931151
|
| Method |
"[Researchers] developed the QconCAT approach for multiplexed
absolute quantification (refs 6, 7). In brief, synthetic genes,
optimized for heterologous expression in Escherichia coli,
encode a single open reading frame that is a concatenation of
tryptic peptides, each of which acts as an internal standard (a
Q-peptide) for a defined protein. Each analyte protein is represented
by at least one, but more preferably two (or more),
Q-peptides." |
| Comments |
"...for Hexokinase 2 (M1 extract 1), [researchers] obtained
5.4 fmol on column (heavy:light ratio, approximately 20:1),
which (combined with the other extracts) gave a total of
123,000 molecules/cell." |
| Entered by |
Uri M |
| ID |
110556 |