Number of copies of hexokinase II

Value 123000 molecules/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Carroll KM, Simpson DM, Eyers CE, Knight CG, Brownridge P, Dunn WB, Winder CL, Lanthaler K, Pir P, Malys N, Kell DB, Oliver SG, Gaskell SJ, Beynon RJ. Absolute quantification of the glycolytic pathway in yeast: deployment of a complete QconCAT approach. Mol Cell Proteomics. 2011 Dec10(12):M111.007633. doi: 10.1074/mcp.M111.007633. p.7 right column bottom paragraphPubMed ID21931151
Method "[Researchers] developed the QconCAT approach for multiplexed absolute quantification (refs 6, 7). In brief, synthetic genes, optimized for heterologous expression in Escherichia coli, encode a single open reading frame that is a concatenation of tryptic peptides, each of which acts as an internal standard (a Q-peptide) for a defined protein. Each analyte protein is represented by at least one, but more preferably two (or more), Q-peptides."
Comments "...for Hexokinase 2 (M1 extract 1), [researchers] obtained 5.4 fmol on column (heavy:light ratio, approximately 20:1), which (combined with the other extracts) gave a total of 123,000 molecules/cell."
Entered by Uri M
ID 110556