Estimated difference in length of nascent polypeptide chains

Value 27 amino acids
Organism Bacteria Escherichia coli
Reference Brandt F, Etchells SA, Ortiz JO, Elcock AH, Hartl FU, Baumeister W. The native 3D organization of bacterial polysomes. Cell. 2009 Jan 23 136(2):261-71. supplementary online material p.6 top paragraphPubMed ID19167328
Method "A novel optical method was built to control the position of the laser trap along the microscope optical axis at 2 nm resolution, facilitating measurement of the force-extension relationship for poly(U)."
Comments "This leads to a total estimate of 81 nucleotides, 54 linking the two ribosomes and 27 nucleotides inside the ribosomes as observed in a T. thermophilus crystal structure (Yusupova et al., 2006). The resulting difference in length of the nascent chains is therefore estimated to be 27 aa’s..."
Entered by Uri M
ID 109077