Diffusion coefficient of EGFP in water

Value 87 μm^2/sec Range: Table - link μm^2/sec
Organism Generic
Reference Arrio-Dupont M, Foucault G, Vacher M, Devaux PF, Cribier S. Translational diffusion of globular proteins in the cytoplasm of cultured muscle cells. Biophys J. 2000 Feb78(2):901-7. p.904 table 1 and right column 2nd paragraphPubMed ID10653802
Method The diffusion of the various proteins was first investigated in buffer A by the MFPP (Modulated fringe pattern photobleaching) technique.
Comments Note that EGFP was very difficult to photobleach, as previously observed for GFP and some of its mutants (Patterson et al., 1997). Nevertheless, the diffusion coefficient estimated, 87µm^2/sec, was in agreement with that determined by fluorescence correlation spectroscopy (Terry et al., 1995).
Entered by Uri M
ID 108250