Mixing time of proteins

Range 0.8 for CIRBP: 2.5 for HMGA2 Generations
Organism Human Homo sapiens
Reference Sigal A et al., Variability and memory of protein levels in human cells. Nature. 2006 Nov 30 444(7119):643-6. p.644 right column 3rd paragraphPubMed ID17122776
Method "To examine the extent and persistence of variability in the levels of different proteins, [researchers] used a fluorescent labelling strategy known as CD-tagging, which tags proteins at their endogenous chromosomal locations [refs 24-26]. A yellow fluorescent protein (YFP) sequence was retrovirally inserted into the genome of human H1299 lung carcinoma cells."
Comments "To compute the mixing time, [researchers] calculated the auto-correlation function A(t) of the protein levels (Fig. 2e, f and see Methods). The mixing time tm was defined as the time when A(t) decayed to one half. [Researchers] found that tm ranged from about 0.8 generations for CIRBP, a protein involved in the cold-shock response, to about 2.5 generations for HMGA2 [a transcription- regulating factor](see Supplementary Information). Furthermore, there was a substantial correlation (R^2˜0.6) between the cell–cell variability in protein levels (CV) and the mixing time (Fig. 3). This correlation between mixing time and variability might pose constraints on possible mechanisms for protein noise (see Supplementary Information)."
Entered by Uri M
ID 107864