Diffusion coefficient of the C-terminal domain of Ada (C-Ada) on elongated DNA

Range 1.3х10^6 Table - link nm^2/sec
Organism Bacteria Escherichia coli
Reference DeSantis MC, Li JL, Wang YM. Protein sliding and hopping kinetics on DNA. Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Feb83(2 Pt 1):021907 p.3 table 1PubMed ID21405863
Primary Source Lin Y, Zhao T, Jian X, Farooqui Z, Qu X, He C, Dinner AR, Scherer NF. Using the bias from flow to elucidate single DNA repair protein sliding and interactions with DNA. Biophys J. 2009 Mar 4 96(5):1911-7. p.1915 left column top paragraphPubMed ID19254550
Method (primary source:) For researchers' single-molecule measurements, they label the C-Ada protein (19 kD) on surface-exposed lysine residues by reaction with Cy 3 NHS ester (GE Healthcare, Piscataway, NJ).
Entered by Uri M
ID 107464