Estimated overall chemical half-life for all mRNAs

Value 6.8 Min
Organism Bacteria Escherichia coli
Reference Selinger DW, Saxena RM, Cheung KJ, Church GM, Rosenow C. Global RNA half-life analysis in Escherichia coli reveals positional patterns of transcript degradation. Genome Res. 2003 Feb13(2):216-23. p.217 left column 2nd paragraphPubMed ID12566399
Method High-density oligonucleotide arrays from Affymetrix were used to study the degradation of RNA over essentially the entire transcriptome of E. coli MG1655. After addition of rifampicin, which prevents initiation of new transcripts by binding to the ß subunit of RNA polymerase (Campbell 2001 PMID 11290327), the total intensity for all mRNAs decreases exponentially with time (R = 0.98) with an estimated overall chemical half-life of 6.8 min.
Comments This is in rough agreement with a reported half-life of 7.5 min for total pulselabeled RNA in comparable conditions (Mohanty and Kushner 1999 PMID 10594833)
Entered by Uri M
ID 106872