Half-lives of Gfp(LAA) and Gfp(LVA)

Value 40 min
Organism Bacteria Escherichia coli
Reference Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S. New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. Appl Environ Microbiol. 1998 Jun64(6):2240-6.PubMed ID9603842
Method Quantitative fluorometer measurements of green fluorescence expressed by the strains derived from E. coli MV1190(?-pir), measured according to T1/2=-ln2/µ where µ=-0.018min-1 is the slope of graph of green fluorescence as function of time after shift from rich medium to minimal medium without inducer.
Entered by Uri M
ID 105186